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human bladder cancer cell lines t24  (ATCC)


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    ATCC human bladder cancer cell lines t24
    Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in <t>T24</t> bladder cancer cells. Cell viability was evaluated at 24 and 48 h time points following exposure to increasing concentrations of cisplatin (0–40 µM). The resulting dose–response curves demonstrate that cisplatin decreases cell viability in a dose- and time-dependent manner. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the corresponding IC 50 values at the respective time points. The lower IC 50 values observed at 48 h of exposure indicate the increasing cytotoxic effect of cisplatin over time.
    Human Bladder Cancer Cell Lines T24, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2772 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+t24+bladder+cancer+cell+line/T24/pmc13203677-47-0-8
    Average 98 stars, based on 2772 article reviews
    human bladder cancer cell lines t24 - by Bioz Stars, 2026-10
    98/100 stars

    Images

    1) Product Images from "Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells"

    Article Title: Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells

    Journal: Biomedicines

    doi: 10.3390/biomedicines14051006

    Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in T24 bladder cancer cells. Cell viability was evaluated at 24 and 48 h time points following exposure to increasing concentrations of cisplatin (0–40 µM). The resulting dose–response curves demonstrate that cisplatin decreases cell viability in a dose- and time-dependent manner. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the corresponding IC 50 values at the respective time points. The lower IC 50 values observed at 48 h of exposure indicate the increasing cytotoxic effect of cisplatin over time.
    Figure Legend Snippet: Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in T24 bladder cancer cells. Cell viability was evaluated at 24 and 48 h time points following exposure to increasing concentrations of cisplatin (0–40 µM). The resulting dose–response curves demonstrate that cisplatin decreases cell viability in a dose- and time-dependent manner. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the corresponding IC 50 values at the respective time points. The lower IC 50 values observed at 48 h of exposure indicate the increasing cytotoxic effect of cisplatin over time.

    Techniques Used:

    Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in 5637 bladder cancer cells. Cells were exposed to varying concentrations of cisplatin for 24 and 48 h, and cell viability was assessed. The dose–response curves indicate a more pronounced sensitivity to cisplatin in 5637 cells. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the calculated IC 50 values. Lower IC 50 values compared to T24 cells demonstrate that this cell line is more sensitive.
    Figure Legend Snippet: Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in 5637 bladder cancer cells. Cells were exposed to varying concentrations of cisplatin for 24 and 48 h, and cell viability was assessed. The dose–response curves indicate a more pronounced sensitivity to cisplatin in 5637 cells. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the calculated IC 50 values. Lower IC 50 values compared to T24 cells demonstrate that this cell line is more sensitive.

    Techniques Used:

    Time-dependent heatmap of autophagy-related gene expression in T24 and 5637 bladder cancer cells following cisplatin treatment. Relative expression levels (2 −ΔΔCt ) are shown for genes involved in autophagy initiation, elongation, selective autophagy, and lysosomal function across 0, 6, 24, and 48 h.
    Figure Legend Snippet: Time-dependent heatmap of autophagy-related gene expression in T24 and 5637 bladder cancer cells following cisplatin treatment. Relative expression levels (2 −ΔΔCt ) are shown for genes involved in autophagy initiation, elongation, selective autophagy, and lysosomal function across 0, 6, 24, and 48 h.

    Techniques Used: Gene Expression, Expressing

    Time-dependent expression profiles of autophagy-related genes in T24 and 5637 bladder cancer cell lines following cisplatin treatment. Relative mRNA expression levels of genes involved in autophagy initiation ( ULK1 , BECN1 ), elongation ( ATG5 , ATG7 , LC3B ), selective autophagy ( p62/SQSTM1 ), and lysosomal regulation (LAMP1 , TFEB ) were evaluated at 0, 6, 24, and 48 h post-treatment. Gene expression levels were calculated using the 2 −ΔΔCt method and are presented as fold changes relative to the control group (0 h). Blue bars represent the T24 cell line, and orange bars represent the 5637 cell line. Data are expressed as mean ± SEM of at least three independent experiments. * p < 0.05, ** p < 0.01 indicating statistical significance between the T24 and 5637 cell lines at the corresponding time points.
    Figure Legend Snippet: Time-dependent expression profiles of autophagy-related genes in T24 and 5637 bladder cancer cell lines following cisplatin treatment. Relative mRNA expression levels of genes involved in autophagy initiation ( ULK1 , BECN1 ), elongation ( ATG5 , ATG7 , LC3B ), selective autophagy ( p62/SQSTM1 ), and lysosomal regulation (LAMP1 , TFEB ) were evaluated at 0, 6, 24, and 48 h post-treatment. Gene expression levels were calculated using the 2 −ΔΔCt method and are presented as fold changes relative to the control group (0 h). Blue bars represent the T24 cell line, and orange bars represent the 5637 cell line. Data are expressed as mean ± SEM of at least three independent experiments. * p < 0.05, ** p < 0.01 indicating statistical significance between the T24 and 5637 cell lines at the corresponding time points.

    Techniques Used: Expressing, Gene Expression, Control

    Related Articles

    Concentration Assay:

    Article Title: Chemotherapy-induced activation of caspase-1 and IL-1α release by cancer cells remotely skews myelopoiesis to drive pro tumorigenic systemic neutrophil-dominant inflammation.
    Article Snippet: .. Human T24 bladder cancer cell line was purchased from ATCC and maintained in DMEM high glucose media (Gibco, 11965092) supplemented with 10% fetal bovine serum (GenDEPOT, F0900-050) and 1x concentration of penicillin-streptomycin (Gibco, 15070063). .. The murine G69 bladder cancer cell line was generated as previously described5 and was maintained in DMEM/F12 media (Gibco, 11320033) supplemented with 10% fetal bovine serum, 1% 100 g/L Dglucose (Gibco, 15023021), 1% sodium pyruvate (Gibco, 160070), 1% NEAA (Gibco, 11140050), 1% penicillin-streptomycin and 18.5 μg/L of carrier-free recombinant mouse EGF (BioLegend, 585608).

    other:

    Article Title: Identification of Hyal2-expressing tumor-associated myeloid cells in cancer: implications for cancer-related inflammation through enhanced hyaluronan degradation
    Article Snippet: The human T24 bladder cancer cell line was purchased from the ATCC (Manassas, VA).

    Article Title: Hyal2 Expression in Tumor-Associated Myeloid Cells Mediates Cancer-Related Inflammation in Bladder Cancer
    Article Snippet: 2 The increased presence of myeloid-derived suppressor cells (MDSC) and tumor-associated macrophages (TAM) in tumor tissue has been extensively reported.. However, their role in the regulation of hyaluronan (HA) metabolism in the tumor microenvironment has not been established.. Here we describe a novel function of tumor-associated myeloid cells related to the enhanced breakdown of extracellular HA in human bladder cancer tissue, leading to the accumulation of small HA fragments with MW <20 kDa.

    Article Title: Adenovirus-mediated LRIG1 expression enhances the chemosensitivity of bladder cancer cells to cisplatin.
    Article Snippet: The human T24 bladder cancer cell line and human QBI-293A embryonic kidney cell line were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

    Cell Culture:

    Article Title: Resveratrol induces apoptosis associated with mitochondrial dysfunction in bladder carcinoma cells.
    Article Snippet: Received 1 March 2011; accepted 18 March 2012.. Objective: Resveratrol shows chemopreventive activity in a variety of human cancers by targeting mitochondria and triggering apoptosis.. The purpose of this study was to investigate the antitumor action of resveratrol in bladder cancer and its underlying mechanism.



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    Image Search Results


    Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in T24 bladder cancer cells. Cell viability was evaluated at 24 and 48 h time points following exposure to increasing concentrations of cisplatin (0–40 µM). The resulting dose–response curves demonstrate that cisplatin decreases cell viability in a dose- and time-dependent manner. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the corresponding IC 50 values at the respective time points. The lower IC 50 values observed at 48 h of exposure indicate the increasing cytotoxic effect of cisplatin over time.

    Journal: Biomedicines

    Article Title: Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells

    doi: 10.3390/biomedicines14051006

    Figure Lengend Snippet: Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in T24 bladder cancer cells. Cell viability was evaluated at 24 and 48 h time points following exposure to increasing concentrations of cisplatin (0–40 µM). The resulting dose–response curves demonstrate that cisplatin decreases cell viability in a dose- and time-dependent manner. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the corresponding IC 50 values at the respective time points. The lower IC 50 values observed at 48 h of exposure indicate the increasing cytotoxic effect of cisplatin over time.

    Article Snippet: Human bladder cancer cell lines T24 and 5637 (ATCC, Manassas, VA, USA) were utilized in this study.

    Techniques:

    Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in 5637 bladder cancer cells. Cells were exposed to varying concentrations of cisplatin for 24 and 48 h, and cell viability was assessed. The dose–response curves indicate a more pronounced sensitivity to cisplatin in 5637 cells. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the calculated IC 50 values. Lower IC 50 values compared to T24 cells demonstrate that this cell line is more sensitive.

    Journal: Biomedicines

    Article Title: Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells

    doi: 10.3390/biomedicines14051006

    Figure Lengend Snippet: Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in 5637 bladder cancer cells. Cells were exposed to varying concentrations of cisplatin for 24 and 48 h, and cell viability was assessed. The dose–response curves indicate a more pronounced sensitivity to cisplatin in 5637 cells. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the calculated IC 50 values. Lower IC 50 values compared to T24 cells demonstrate that this cell line is more sensitive.

    Article Snippet: Human bladder cancer cell lines T24 and 5637 (ATCC, Manassas, VA, USA) were utilized in this study.

    Techniques:

    Time-dependent heatmap of autophagy-related gene expression in T24 and 5637 bladder cancer cells following cisplatin treatment. Relative expression levels (2 −ΔΔCt ) are shown for genes involved in autophagy initiation, elongation, selective autophagy, and lysosomal function across 0, 6, 24, and 48 h.

    Journal: Biomedicines

    Article Title: Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells

    doi: 10.3390/biomedicines14051006

    Figure Lengend Snippet: Time-dependent heatmap of autophagy-related gene expression in T24 and 5637 bladder cancer cells following cisplatin treatment. Relative expression levels (2 −ΔΔCt ) are shown for genes involved in autophagy initiation, elongation, selective autophagy, and lysosomal function across 0, 6, 24, and 48 h.

    Article Snippet: Human bladder cancer cell lines T24 and 5637 (ATCC, Manassas, VA, USA) were utilized in this study.

    Techniques: Gene Expression, Expressing

    Time-dependent expression profiles of autophagy-related genes in T24 and 5637 bladder cancer cell lines following cisplatin treatment. Relative mRNA expression levels of genes involved in autophagy initiation ( ULK1 , BECN1 ), elongation ( ATG5 , ATG7 , LC3B ), selective autophagy ( p62/SQSTM1 ), and lysosomal regulation (LAMP1 , TFEB ) were evaluated at 0, 6, 24, and 48 h post-treatment. Gene expression levels were calculated using the 2 −ΔΔCt method and are presented as fold changes relative to the control group (0 h). Blue bars represent the T24 cell line, and orange bars represent the 5637 cell line. Data are expressed as mean ± SEM of at least three independent experiments. * p < 0.05, ** p < 0.01 indicating statistical significance between the T24 and 5637 cell lines at the corresponding time points.

    Journal: Biomedicines

    Article Title: Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells

    doi: 10.3390/biomedicines14051006

    Figure Lengend Snippet: Time-dependent expression profiles of autophagy-related genes in T24 and 5637 bladder cancer cell lines following cisplatin treatment. Relative mRNA expression levels of genes involved in autophagy initiation ( ULK1 , BECN1 ), elongation ( ATG5 , ATG7 , LC3B ), selective autophagy ( p62/SQSTM1 ), and lysosomal regulation (LAMP1 , TFEB ) were evaluated at 0, 6, 24, and 48 h post-treatment. Gene expression levels were calculated using the 2 −ΔΔCt method and are presented as fold changes relative to the control group (0 h). Blue bars represent the T24 cell line, and orange bars represent the 5637 cell line. Data are expressed as mean ± SEM of at least three independent experiments. * p < 0.05, ** p < 0.01 indicating statistical significance between the T24 and 5637 cell lines at the corresponding time points.

    Article Snippet: Human bladder cancer cell lines T24 and 5637 (ATCC, Manassas, VA, USA) were utilized in this study.

    Techniques: Expressing, Gene Expression, Control